Regan Iseen/vine in Normal Human Sera
نویسندگان
چکیده
polymerized antibody for 1 hr and subsequently centrifuged. The pellet containing the enzymatically active enzyme-anti body complex is immediately assayed for enzyme activity with 72 mM phenylphosphate as substrate. Control tubes contain ing 0.15 M NaCl instead of inactivated serum were carried out through the procedure to check on the spontaneous hydrolysis of phenylphosphate at alkaline pH values.
منابع مشابه
A sensitive immunochemical method for the determination of the Regan isoenzyme in serum.
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